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JAC Advance Access originally published online on April 1, 2008
Journal of Antimicrobial Chemotherapy 2008 62(1):133-136; doi:10.1093/jac/dkn145
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© The Author 2008. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Original research

Dissemination of ESBL and Qnr determinants in Enterobacter cloacae in Algeria

Hassen Iabadene1, Yamina Messai1, Houria Ammari2, Nadjia Ramdani-Bouguessa3, Saliha Lounes1, Rabah Bakour1 and Guillaume Arlet4,*

1 Laboratoire de Biologie Cellulaire et Moléculaire, Faculté des Sciences Biologiques, Université des Sciences et de la Technologie Houari Boumediene, BP 32 El-Alia, Bab-Ezzouar 16111, Alger, Algérie 2 Laboratoire de Bactériologie, CHU Beni Messous, Alger, Algérie 3 Service de Microbiologie, CHU Mustapha Bacha, Alger, Algérie 4 Université Paris VI, Faculté de Médecine Pierre et Marie Curie, Laboratoire de Bactériologie, UPRES EA 2392, Paris, France

Received 18 January 2008; returned 31 January 2008; revised 10 March 2008; accepted 10 March 2008

* Correspondence address. Service de Bactériologie-Hygiène, Hôpital Tenon, 4 rue de la Chine, 75970 Paris cedex 20, France. Tel: +33-1-56-01-70-18; Fax: +33-1-56-01-61-08; E-mail: guillaume.arlet{at}tnn.aphp.fr

Objectives: The aim of this study is to evaluate the prevalence and diversity of extended-spectrum β-lactamases (ESBLs) in Enterobacter cloacae clinical isolates collected from Algerian hospitals and to verify the association with qnr genes.

Methods: MICs were determined by Etest for isolates giving positive double-disc synergy tests, and all isolates were screened by PCR and sequenced, respectively, for blaTEM, blaCTX-M, blaSHV and blaVEB genes and for qnr genes (qnrA, qnrB, qnrS), using specific primers.

Results: The prevalence of ESBLs was 25/141 (17.7%) with 11, 9, 4 and 1 isolates testing positive for genes encoding CTX-M-15, CTX-M-3, SHV-12 and VEB-1, respectively. Two SHV-12 producers and one CTX-M-15 producer expressed QnrS1, one isolate produced CTX-M-15 and QnrB1 and one SHV-12 producer co-expressed QnrS1 and QnrB4. qnrA was not detected in our collection, and qnr alleles were not detected in non-ESBL-producing isolates.

Conclusions: SHV-12, QnrS1, QnrB1 and QnrB4 were reported for the first time in Algeria. This study also described a co-expression of qnrS1 and qnrB4 by an SHV-12 producer isolate.

Keywords: E. cloacae , CTX-M , SHV-12 , QnrB , QnrS


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